Project Overview
Bullseye Biotechnologies is a pioneer in the development of vascularized cardiac organoids, which have applications in drug discovery and tissue engineering therapies. Their groundbreaking work on self-assembled vascularized heart organoids—recently published in Science (DOI: 10.1126/science.adu9375)—represents a major advance in modeling early human development.
Mosaic partnered with Bullseye to translate their research protocols into a manufacturable product, developing automated liquid handling schemes and production workflows for pilot-scale manufacturing of their stem cell differentiation kit.
Technical Solution
Automated Production on OPENTRONS OT-2
- Reagent production: Automated preparation of differentiation media with precise volumetric control
- Plate production: Automated coating and patterning of micro-patterned multi-well plates
- Quality control: Integrated validation steps to ensure batch-to-batch consistency
- Protocol optimization: Iterative refinement to maximize throughput and minimize waste
Kit Manufacturing & Distribution
- Packaging design: Developed commercial packaging maintaining reagent stability during shipping
- Component integration: Coordinated reagents, plates, and protocols into cohesive kits
- Test kit distribution: Managed distribution to academic partners for validation
- Documentation: Created user protocols and quality specifications
Biological Validation
While Mosaic’s primary expertise lies in instrumentation, we leveraged our in-house capabilities for this project—including liquid handling expertise and access to BSL-2 facilities at UC Berkeley for validation with induced pluripotent stem cells.
Impact
The automation enabled Bullseye to move from manual bench-scale to pilot manufacturing, improving consistency and reducing costs while accelerating distribution to research partners. This project demonstrates Mosaic’s capability to bridge cutting-edge biological research and practical manufacturing solutions—supporting life science companies at the critical transition from research to product.
Developing a life science product that requires automation or manufacturing scale-up? Contact us to discuss how we can help translate your research into a manufacturable product.